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KMID : 0613819990090010008
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Journal of Life Science
1999 Volume.9 No. 1 p.8 ~ p.14
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Antifugal Activity and Cultural Characteristics of the Streptomyces sp. A252.
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Abstract
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The growth rate of the A252 strain was increased in tryptic soy broth (TSB) and malt extract-yeast extract medium (ISP-2), but the antifungal activity of culture filtrate was efficient in the media of TSB and nutrient broth. The mycelial growth and the antifungal activity of culture filtrate in TSB medium were optimized at 25$\circ$C and pH 6.5. The growth in 2% TSB concentration was more effective than 1%, but there was no difference of the antifungal activity by the TSB concentrations. The mycelial growth of A252 strain reached to maximum at 72 hr after inoculation, whereas the antifungal activity of culture filtrate was shown to have the highest level at idiophase (60 hr) after inoculation and was decreased a little after 96 hr incubation. The antifungal activity was stable in the pH range of 4 to 11 and evenly at 121$\circ$C . The A252 strain was characterized as Streptomyces species by the physiological properties and examination of sporophore morphology.
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